Purification and Properties of HeLa Cell Phosphorylase
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چکیده
منابع مشابه
Purification and properties of HeLa cell plasma membranes.
A simple method for the preparation of HeLa cell plasma membrane ghosts without the use of cross-linking reagents or Cxatives has been developed. The membranes have been characterized by phase and electron microscopy and by the use of radioactive precursors to RNA, protein, glycoprotein, and lipid. The glycoprotein and polypeptide constituents have been analyzed by sodium dodecyl sulfate-acryla...
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A procedure for the purification of rabbit liver phosphorylase phosphatase is described. The specific activity of the preparation is 2,100 units/mg of protein, representing a 25,000-fold purification. During the initial steps of the purification a large activation of enzyme activity was observed. The molecular weight of the purified enzyme was estimated by Sephadex G-75 chromatography to be 35,...
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A method for the purification of polynucleotide phosphorylase from Escherichia coli has been developed. The purified enzyme has a specific activity 700-fold higher than the crude extract. When enzyme fractions obtained at different stages of the purification were assayed by phosphorolysis of polyadenylic acid (poly A) or 32P-orthophosphate exchange with the S’diphosphates of adenosine, uridine,...
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Purine nucleoside phosphorylase (inosine + Pi ti hypoxanthine + a-D-ribose l-phosphate, EC 2.4.2.1) from Salmonella typhimurium LT-2 has been purified 230-fold. High speed sedimentation equilibrium studies showed that the molecular weight of the native enzyme was 141,000. A molecular weight of 130,000 i 10% was determined by Sephadex G-150 filtration. Disc gel electrophoresis of the enzyme in t...
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1. Polynucleotide phosphorylase was partially purified from the inner membrane of rat liver mitochondria. 2. The partially purified particulate enzyme catalyses phosphorolysis of poly(A), poly(C), poly(U) and RNA to nucleoside diphosphates. 3. It is devoid of nucleoside diphosphate-polymerization activity. 4. Variable amounts of ADP/P(i)-exchange activity are associated with the polynucleotide ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91184-1